hig.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard-cite-them-right
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • sv-SE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • de-DE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
The genetics of antibiotic production and the role of antibiotics in biological control of Erwinia amylovora by Erwinia herbicola
Cornell University.
1997 (engelsk)Doktoravhandling, monografi (Annet vitenskapelig)
Abstract [en]

Erwinia herbicola is an epiphyte of apple and pear with potential as a biocontrol agent of Erwinia amylovora, which causes fire blight. This research aimed to assess the relative role of antibiotics produced by E. herbicola, Eh318 as a mechanistic basis for biocontrol. A genomic library of Eh318 DNA was constructed in Escherichia coli and two distinct cosmids, pCPP702 and pCPP704, were identified that conferred upon E. coli the ability to produce two antibiotics inhibitory to E. amylovora. The antibiotics were distinct based on their spectra of activity, differential susceptibility to the presence of histidine and arginine and antibiotic production by marker-exchange mutants of Eh318. Transposon mutagenesis and subcloning were used to delineate the Eh318 DNA that enabled E. coli to produce the two antibiotics. The smallest clone that conferred antibacterial activity was pCPP717. Its antibiotic was named pantocin A. The Eh318 insert DNA of pCPP717 revealed three predicted genes, paaA, paaB and paaC, in a 2.7 kb region. The predicted paaA gene product is similar in sequence to a group of biosynthetic enzymes that possess a dinucleotide binding motif. PaaC was judged to encode a membrane protein. The second antibiotic was named pantocin B. Its synthesis is conferred on E. coli by DNA harbored in clone pCPP719. Between 19 kb and 20 kb of Eh318 DNA is needed for the production of pantocin B. Direct Tn5- and marker-exchange mutants of Eh318, deficient in pantocin A and/or pantocin B, were created. The mutant strains were tested for biocontrol ability in immature pear fruit in the laboratory and in apple blossoms in a controlled environment chamber. Results from both assays revealed that the marker-exchange mutant deficient in both antibiotics (Eh440) protected against fire blight less well than Eh318. The single marker-exchange mutants, Eh421 (deficient in pantocin A) and Eh439 (deficient in pantocin B), were not significantly impaired in biocontrol ability, whereas three directly induced Tn5-mutants, Eh454, Eh464 and Eh468, were less effective than Eh318. Thus, pantocins contribute to but are not solely responsible for the biological control of fire blight by E. herbicola Eh318.

sted, utgiver, år, opplag, sider
Ann Arbor: Cornell University , 1997. , s. xiii, 294s. 307-307 p.
Emneord [en]
Biological sciences, fire blight, pantocin, Plant pathology, Microbiology, Molecular biology, 0480:Plant pathology, 0307:Molecular biology, 0410:Microbiology
HSV kategori
Identifikatorer
URN: urn:nbn:se:hig:diva-20753ISBN: 9780591434477 (tryckt)ISBN: 0591434474 (tryckt)OAI: oai:DiVA.org:hig-20753DiVA, id: diva2:875751
Merknad

9733679

Tilgjengelig fra: 2015-12-01 Laget: 2015-12-01 Sist oppdatert: 2018-03-13bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

http://search.proquest.com/docview/304340077http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:9733679

Personposter BETA

Wright, Sandra A. I.

Søk i DiVA

Av forfatter/redaktør
Wright, Sandra A. I.

Søk utenfor DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric

isbn
urn-nbn
Totalt: 608 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard-cite-them-right
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • sv-SE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • de-DE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf