Background: High occupational stress is a common phenomenon today, especially for health care workers. Because of the nature of the nursing profession, nurses directly confront severe illness, pain, grief, and death. Therefore, taking measures to relieve the nurses’ working stress needs us to pay attention to.

Aim: To evaluate the working stress among registered nurses in a hospital.

Method: Convenience sampling was used and 100 registered nurses from four departments completed the questionnaire. The Stress Overload Scale (SOS) and the demographic questionnaire were used to investigate the nurse’s occupational stress in the form of an electronic questionnaire. And SPSS (23.0) was used to analyze data.

Result: The mean score of SOS was 66.8±15.19 points, which was in the middle level. According to the analysis of the “Stress Risk Assessment Table”, “High Risk” accounted for 29%, “Low Risk” 38%, “Challenging” 30%, and “Fragile” 3%. It showed that the medical department had the highest working stress level in four departments.

Conclusions: Nurses were under a certain amount of stress in their work, and the medical department had the highest working stress level in four departments. According to the “Stress Risk Assessment Table”, “Low Risk” accounted for the highest proportion, at 38%. The SOS scale could help them to know stress levels and then took methods to reduce them.