Objective: This study documented the temporal patterns of sitting, non-sitting and time-in-bed (TIB) of Brazilian office workers working from home during the pandemic; and also determined the extent to which these patterns differed between normal-weight (NWW) and overweight workers (OWW). Methods: Behaviors were monitored over 5 days using accelerometers in 33 NWW (BMI <25 kg/m2) and 40 OWW (BMI ≥25 kg/m2). Time-use compositions were described in terms of sitting, non-sitting and TIB. Temporal patterns of sitting/non-sitting were quantified according to Exposure Variation Analysis into short (≤5 min), moderate (>5 and ≤20) and long uninterrupted bouts (>20). Following a compositional data analysis, isometric log-ratios (ilr) were calculated; ilr1–TIB relative to time spent awake, ilr2–sitting (all bouts) relative to non-sitting (all bouts), ilr3–sitting in short relative to moderate and long bouts, ilr4–sitting in moderate relative to long bouts; ilr5 and ilr6 represent the same behavior contrasts as ilr3 and ilr4, but for non-sitting. We examined differences between groups using MANOVA, followed by univariate post-hoc tests of pairwise differences. Results: NWW spent more time sitting in short bouts (50 min) and less time in moderate and long bouts (154 and 552) than OWW (42, 155 and 585). For non-sitting, NWW spent more time in short and moderate bouts (71 and 96) and less time in long bouts (45) than OWW (60, 83 and 54). NWW had longer TIB (473) than OWW (461). NWW and OWW differed in the set of ilrs as a whole (p=0.05). The post-hoc tests showed that time spent sitting in short relative to longer bouts (ilr3) was smaller for OWW than for NWW (p=0.05). This indicates that OWW had less variation in sitting behaviors. Conclusions: OWW spent less time at work sitting in short uninterrupted bouts, relative to sitting for longer bouts, than NWW, while the relative time-use did not differ for other behaviors.