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Nitrite metabolism in Debaryomyces hansenii TOB-Y7, a yeast strain involved in tobacco fermentation
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2007 (English)In: Applied Microbiology and Biotechnology, ISSN 0175-7598, E-ISSN 1432-0614, Vol. 75, no 3, p. 633-645Article in journal (Refereed) Published
Abstract [en]

The Italian cigar manufacturing process includes a fermentation step that leads to accumulation of nitrite and tobacco-specific nitrosamines (TSNA), undesirable by-products due to their negative impact on health. In this study, growth and biochemical properties of Debaryomyces hansenii TOB-Y7, a yeast strain that predominates during the early phase of fermentation, have been investigated. With respect to other D. hansenii collection strains (Y7426, J26, and CBS 1796), TOB-Y7 was characterized by the ability to tolerate very high nitrite levels and to utilize nitrite, but not nitrate, as a sole nitrogen source in a chemically defined medium, a property that was enhanced in microaerophilic environment. The ability to assimilate nitrite was associated to the presence of YNI1, the gene encoding the assimilatory NAD(P)H:nitrite reductase (NiR), absent in Y7426, J26, and CBS 1796 by Southern blot data. YNI1 from TOB-Y7 was entirely sequenced, and its expression was analyzed in different media by Northern blot and reverse transcriptase polymerase chain reaction. The evidence that, in D. hansenii TOB-Y7, YNI1 was transcriptional active also in the presence of high ammonia concentration typical of tobacco fermentation, stimulated the development of an improved process that, on a laboratory scale, was proved to be effective in minimizing nitrite and TSNA accumulation.

Place, publisher, year, edition, pages
Springer , 2007. Vol. 75, no 3, p. 633-645
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Biological Sciences
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URN: urn:nbn:se:hig:diva-43557DOI: 10.1007/s00253-007-0867-2OAI: oai:DiVA.org:hig-43557DiVA, id: diva2:1825241
Available from: 2024-01-09 Created: 2024-01-09 Last updated: 2024-01-09Bibliographically approved

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Silvestro, Daniele

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CiteExportLink to record
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Citation style
  • apa
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  • Other style
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  • sv-SE
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  • en-US
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  • de-DE
  • Other locale
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Output format
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  • asciidoc
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