In this paper an approach to develop a non-invasive glucose measured system based on the best linear approximation (BLA) and electrical impedance spectroscopy (EIS) is presented. In order to test the advantages of the technique, three blood components at reference blood concentration are tested. In these solutions the level of glucose was varied from normal to pathological concentrations. The results show the ability of BLA-EIS to detect the glucose changes by combining the amplitude and phase behavior of the impedance.