With the development of network and information technology, E-mail has became increasingly popular and the society’s indispensable needs. However, virus spreading via the email are also increasing. The Email attachment, such as, PDF documents, EXE programs, can spread viruses from one computer to another computer. PDF documents are one of the widely used reading or sharing documents, and the attackers are using malicious PDF documents increasingly. In this paper, in order to detect the viruses in the PDF document, I have analyzed the file structure, document structure and objects. I select two methods PDF Scrutinizer and MDScan to scan the PDF document viruses.