hig.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard-cite-them-right
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • sv-SE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • de-DE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Considerations on Circuit Design and Data Acquisition of a Portable Surface Plasmon Resonance Biosensing System
Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
Department of Electrical Engineering, Henan Agricultural University, Zhengzhou, China.
Show others and affiliations
2015 (English)In: Sensors, ISSN 1424-8220, E-ISSN 1424-8220, Vol. 15, no 8, 20511-20523 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to develop a circuit for an inexpensive portable biosensing system based on surface plasmon resonance spectroscopy. This portable biosensing system designed for field use is characterized by a special structure which consists of a microfluidic cell incorporating a right angle prism functionalized with a biomolecular identification membrane, a laser line generator and a data acquisition circuit board. The data structure, data memory capacity and a line charge-coupled device (CCD) array with a driving circuit for collecting the photoelectric signals are intensively focused on and the high performance analog-to-digital (A/D) converter is comprehensively evaluated. The interface circuit and the photoelectric signal amplifier circuit are first studied to obtain the weak signals from the line CCD array in this experiment. Quantitative measurements for validating the sensitivity of the biosensing system were implemented using ethanol solutions of various concentrations indicated by volume fractions of 5%, 8%, 15%, 20%, 25%, and 30%, respectively, without a biomembrane immobilized on the surface of the SPR sensor. The experiments demonstrated that it is possible to detect a change in the refractive index of an ethanol solution with a sensitivity of 4.99838 × 10(5) ΔRU/RI in terms of the changes in delta response unit with refractive index using this SPR biosensing system, whereby the theoretical limit of detection of 3.3537 × 10(-5) refractive index unit (RIU) and a high linearity at the correlation coefficient of 0.98065. The results obtained from a series of tests confirmed the practicality of this cost-effective portable SPR biosensing system.

Place, publisher, year, edition, pages
2015. Vol. 15, no 8, 20511-20523 p.
Keyword [en]
ADC conversion time, biosensing system, linear CCD array, microcontroller, refractive index, surface plasmon resonance
National Category
Other Electrical Engineering, Electronic Engineering, Information Engineering Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hig:diva-20199DOI: 10.3390/s150820511ISI: 000360906500137PubMedID: 26295398Scopus ID: 2-s2.0-84939824326OAI: oai:DiVA.org:hig-20199DiVA: diva2:851063
Note

Funding Agency:

Grant Number National Natural Science Foundation of China  Grant no: U1304305 

State Key Laboratory of Wheat and Maize Crop Science Grant no: SKL2014ZH-06 

Henan Province Science and Technology Cooperation Program Grant no: 132106000073 

Available from: 2015-09-03 Created: 2015-09-03 Last updated: 2016-02-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus
By organisation
Department of Electronics, Mathematics and Natural Sciences
In the same journal
Sensors
Other Electrical Engineering, Electronic Engineering, Information EngineeringBiomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 36 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard-cite-them-right
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • sv-SE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • de-DE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf